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Localization of a determinant mediating partial macrolide resistance in Staphylococcus aureus
M Matsuoka1, K Endou, Y Nakajima
1Division of Microbiology, Hokkaido Institute of Pharmaceutical Sciences.
Microbiology and Immunology
|January 1, 1990
Summary
Four Staphylococcus aureus strains exhibited resistance to macrolide antibiotics, except tylosin and rokitamycin. This macrolide resistance determinant was found to be located on the bacterial chromosome.
Area of Science:
- Microbiology
- Molecular Biology
- Antibiotic Resistance
Background:
- Staphylococcus aureus is a significant human pathogen.
- Macrolide antibiotics are crucial for treating S. aureus infections.
- Understanding antibiotic resistance mechanisms is vital for public health.
Purpose of the Study:
- To investigate the characteristics of macrolide-resistant Staphylococcus aureus strains.
- To determine the genetic location of the macrolide resistance determinant.
Main Methods:
- Isolation and characterization of S. aureus strains.
- Agarose gel electrophoresis and CsCl-ethidium bromide density gradient analysis.
- Bacteriophage transduction experiments.
Main Results:
- Four S. aureus strains showed constitutive resistance to macrolides (excluding tylosin and rokitamycin).
- Resistance was specific, with susceptibility to lincosamides and streptogramin B.
- Genetic analysis indicated the resistance determinant resides on the bacterial chromosome.
Conclusions:
- A novel chromosomal macrolide resistance mechanism in S. aureus was identified.
- This finding contributes to understanding the evolution of antibiotic resistance.
- Further research is needed to elucidate the specific molecular basis of this resistance.