Related Experiment Video

Updated: Jun 5, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Passive optical separation and enrichment of cells by size difference

Siew-Kit Hoi, Vuong Hoang Kim, Nguyen Manh Huy

    Biomicrofluidics
    |January 26, 2011
    PubMed
    Summary

    This study introduces a microfluidic device for cell sorting using optical force. The system efficiently separates yeast cells by size, yielding high-purity samples of smaller cells.

    More Related Videos

    Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function09:25

    Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

    Published on: February 16, 2017

    Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination08:52

    Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

    Published on: February 18, 2017

    Related Concept Videos

    Overview Of Cell Separation And Isolation01:20

    Overview Of Cell Separation And Isolation

    Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
    Size-Exclusion Chromatography01:08

    Size-Exclusion Chromatography

    In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
    Silica particles offer advantages such as rigidity,...
    Centrifugation01:05

    Centrifugation

    Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
    Subcellular Fractionation01:32

    Subcellular Fractionation

    The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
    Differential Centrifugation
    Differential centrifugation is...
    Flow Cytometry01:23

    Flow Cytometry

    The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
    In...