Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 5, 2026

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
09:20

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

Simple surface modification of poly(dimethylsiloxane) for DNA hybridization.

Jinwen Zhou1, Nicolas H Voelcker, Amanda V Ellis

  • 1Centre for NanoScale Science and Nanotechnology, School of Chemical and Physical Sciences, Flinders University, Adelaide SA 5001, Australia.

Biomicrofluidics
|January 26, 2011
PubMed
Summary

We developed a simple method to modify poly(dimethylsiloxane) (PDMS) surfaces for DNA attachment. This technique creates a functionalized PDMS material capable of specific DNA hybridization, useful for biosensor applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Micropillar-Based Biosensor for Monitoring Contamination- and Compound-Induced Metabolic States in Cell Cultures.

ACS applied materials & interfaces·2026
Same author

Light-Activated Isolation of High-Quality Mitochondria for Therapeutic Transplantation.

Angewandte Chemie (International ed. in English)·2026
Same author

Quantification of microplastics in soil and sediments using dry ice assisted fractionation with an Agilent 8700 laser direct infrared chemical imaging system.

Analytical methods : advancing methods and applications·2026
Same author

Poking Pluripotency: Nanoinjection Into Human iPSCs.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Hybrid macrophage-mitochondria extracellular vesicles for mitochondrial ROS regulation in diabetic wounds.

Nature communications·2026
Same author

Electrochemical β-Lactam Probes for Rapid Detection of β-Lactamases: From Solution-Phase Activation toward Surface-Integrated Biosensing.

Journal of the American Chemical Society·2026

Area of Science:

  • Materials Science
  • Surface Chemistry
  • Biotechnology

Background:

  • Poly(dimethylsiloxane) (PDMS) is a versatile polymer widely used in microfluidics and biosensors.
  • Functionalizing PDMS surfaces is crucial for immobilizing biomolecules like DNA.
  • Existing methods for PDMS surface modification can be complex or lack robust biomolecule attachment.

Purpose of the Study:

  • To present a straightforward chemical modification of PDMS for creating carboxyl-terminated surfaces.
  • To demonstrate the covalent attachment of DNA oligonucleotides to the modified PDMS.
  • To validate the functionality of the immobilized DNA for hybridization assays.

Main Methods:

  • Curing a mixture of undecylenic acid (UDA) in PDMS prepolymer on a gold-coated glass slide pretreated with 3-mercaptopropionic acid (MPA).

More Related Videos

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Published on: June 23, 2016

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
08:56

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Published on: July 17, 2018

Related Experiment Videos

Last Updated: Jun 5, 2026

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
09:20

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Published on: June 23, 2016

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
08:56

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Published on: July 17, 2018

  • Utilizing surface energy minimization for diffusion of UDA carboxyl groups to the surface during curing.
  • Peeling the cured PDMS to expose interfacial carboxyl groups for subsequent amide linkage with amino-terminated DNA oligonucleotides.

    • Main Results:

    • Successfully generated PDMS surfaces with accessible carboxyl groups after curing and peeling.
    • Demonstrated covalent tethering of 5'-amino terminated DNA oligonucleotides via amide bonds.
    • Confirmed successful hybridization of fluorescein-labeled complementary DNA to the immobilized oligonucleotides using fluorescence microscopy.

    Conclusions:

    • The described method provides a simple and effective way to chemically modify PDMS surfaces.
    • The modified PDMS surfaces support robust covalent immobilization of DNA oligonucleotides.
    • This functionalized PDMS material is suitable for applications requiring specific DNA-DNA hybridization, such as in biosensing.