Related Experiment Video
Updated: Jun 5, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Archaeal promoter architecture and mechanism of gene activation
Nan Peng1, Xiang Ao, Yun Xiang Liang
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
Abstract:
Sulfolobus solfataricus and Sulfolobus islandicus contain several genes exhibiting D-arabinose-inducible expression and these systems are ideal for studying mechanisms of archaeal gene expression. At sequence level, only two highly conserved cis elements are present on the promoters: a regulatory element named ara box directing arabinose-inducible expression and the basal promoter element TATA, serving as the binding site for the TATA-binding protein. Strikingly, these promoters possess a modular structure that allows an essentially inactive basal promoter to be strongly activated. The invoked mechanisms include TFB (transcription factor B) recruitment by the ara-box-binding factor to activate gene expression and modulation of TFB recruitment efficiency to yield differential gene expression.
Related Concept Videos
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
RNA Polymerase II Accessory Proteins
Prokaryotic Gene Structure and Organization

