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Updated: May 14, 2026

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
Published on: July 6, 2021
FRET (fluorescence resonance energy transfer) sheds light on transcription
Dina Grohmann1, Daniel Klose, Daniel Fielden
1RNAP Laboratory, UCL Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK. d.grohmann@ucl.ac.uk
Abstract:
The complex organization of the transcription machinery has been revealed mainly by biochemical and crystallographic studies. X-ray structures describe RNA polymerases and transcription complexes on an atomic level, but fail to portray their dynamic nature. The use of fluorescence techniques has made it possible to add a new layer of information to our understanding of transcription by providing details about the structural rearrangement of mobile elements and the network of interactions within transcription complexes in solution and in real-time.
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