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Updated: Jun 5, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
The Pseudomonas aeruginosa DNR transcription factor: light and shade of nitric oxide-sensing mechanisms
Giorgio Giardina1, Nicoletta Castiglione, Manuela Caruso
1Department of Biochemical Sciences A. Rossi Fanelli, University of Rome Sapienza, P. e Aldo Moro, n. 5, 00185, Rome, Italy.
Abstract:
In response to environmental conditions, NO (nitric oxide) induces global changes in the cellular metabolism of Pseudomonas aeruginosa, which are strictly related to pathogenesis. In particular, at low oxygen tensions and in the presence of NO the denitrification alternative respiration is activated by a key regulator: DNR (dissimilative nitrate respiration regulator). DNR belongs to the CRP (cAMP receptor protein)-FNR (fumarate and nitrate reductase regulatory protein) superfamily of bacterial transcription factors. These regulators are involved in many different pathways and distinct activation mechanism seems to be operative in several cases. Recent results indicate that DNR is a haem protein capable of discriminating between NO and CO (carbon monoxide). On the basis of the available structural data, a suggested activation mechanism is discussed.
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