[Level of DNA adduct in lung cancer tissues and its influencing factors]
Zhi Zhang1, Xuemei Zhang, Wenmin Yang
1Department of Medical Oncology, Tangshan Worker Hospital, Hebei Medical University, Tangshan, Hebei 063000, P.R.China.
Background:
To detect DNA adduct in lung cancer tissues and analyze the factors influencing formation of DNA adduct.
Methods:
DNA was extracted from the tumor tissues derived from 34 patients with lung cancer. DNA adducts were analyzed using 32P-postlabeling method with P 1 nuclear modification.
Results:
DNA adduct level was significantly higher in smoking patients than that in non smoking patients(P < 0.05). DNA adduct level was related to the histological classification, degree of cell differentiation and lymph node metastasis, but not to age and sex.
Conclusions:
DNA adduct level might be used as a potential marker to estimate malignant degree for lung cancer.
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