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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
What is Population Genetics?01:25

What is Population Genetics?

A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.While some alleles of a given gene might be observed commonly, other variants...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Collection and Extraction of Saliva DNA for Next Generation Sequencing
06:58

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

RADSeq: next-generation population genetics.

John W Davey1, John L Davey, Mark L Blaxter

  • 1Institute of Evolutionary Biology, Edinburgh, UK. john.davey@ed.ac.uk

Briefings in Functional Genomics
|January 27, 2011
PubMed
Summary
This summary is machine-generated.

Restriction-site associated DNA sequencing provides high-resolution population genomic data for any organism affordably. This method enables genetic diversity analysis in wild and non-model species, advancing ecological population genomics.

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Last Updated: Jun 5, 2026

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Published on: August 27, 2014

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Area of Science:

  • Genomics
  • Population Genetics
  • Molecular Biology

Background:

  • Next-generation sequencing (NGS) significantly impacts biological research, particularly in analyzing population genetic diversity.
  • Genome-scale population genetic studies have historically been limited to well-funded model organisms due to high costs.
  • There is a need for cost-effective methods to study genetic diversity in a wider range of species.

Purpose of the Study:

  • To introduce and highlight the utility of Restriction-site associated DNA sequencing (RADseq) for population genomics.
  • To demonstrate RADseq as a method for obtaining high-resolution population genomic data across diverse organisms.
  • To showcase RADseq's potential for ecological population genomics applications.

Main Methods:

  • Restriction-site associated DNA sequencing (RADseq) involves reduced complexity genome sampling.
  • This method sequences thousands of markers across numerous individuals.
  • RADseq is applicable to both wild populations and non-traditional study species.

Main Results:

  • RADseq enables high-resolution population genomic data generation at reasonable costs.
  • The technology has been successfully applied to wild populations and non-model species.
  • Thousands of genetic markers can be sequenced per individual using RADseq.

Conclusions:

  • Restriction-site associated DNA sequencing is a powerful and accessible tool for population genomics.
  • RADseq democratizes genome-scale population genetic studies beyond well-funded model systems.
  • This method is poised to become a key technology in ecological population genomics research.