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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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Related Experiment Video

Updated: Jun 5, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Next generation sequencing based approaches to epigenomics.

Martin Hirst1, Marco A Marra

  • 1Genome Sciences Centre, Vancouver, BC, Canada. mhirst@bcgsc.ca

Briefings in Functional Genomics
|January 27, 2011
PubMed
Summary
This summary is machine-generated.

Next generation sequencing enables detailed epigenomic studies. This review covers essential methods for profiling the epigenome, focusing on standardization and quality control for reliable results.

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Last Updated: Jun 5, 2026

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Area of Science:

  • Epigenomics
  • Genomics
  • Molecular Biology

Background:

  • Next-generation sequencing (NGS) has revolutionized epigenomic research.
  • High-resolution epigenome profiling is now achievable.
  • The field is advancing towards method standardization and quality control.

Purpose of the Study:

  • To review methodologies for epigenome profiling using NGS.
  • To discuss key aspects of library preparation, sequencing platforms, and analysis techniques.

Main Methods:

  • Review of existing literature on epigenomic profiling techniques.
  • Focus on NGS-based methods.
  • Discussion of library preparation, sequencing, and data analysis.

Main Results:

  • Comprehensive overview of current NGS-based epigenomic profiling methods.
  • Identification of critical steps requiring standardization.
  • Emphasis on quality control measures.

Conclusions:

  • NGS technologies are pivotal for modern epigenomics.
  • Standardization and rigorous quality control are crucial for advancing the field.
  • This review provides a guide to current methodologies.