Related Experiment Video

Updated: Jun 5, 2026

Establishment of an Extracellular Acidic pH Culture System
09:41

Establishment of an Extracellular Acidic pH Culture System

Published on: November 19, 2017

A clinical approach to the diagnosis of Acid-base disorders

R A Bear

    Canadian Family Physician Medecin De Famille Canadien
    |January 27, 2011
    PubMed

    More Related Videos

    Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells10:18

    Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

    Published on: June 13, 2019

    Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate06:47

    Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

    Published on: December 12, 2015

    Related Concept Videos

    Diagnosing Acidosis and Alkalosis01:24

    Diagnosing Acidosis and Alkalosis

    Diagnosing acid-base imbalances involves systematically analyzing arterial blood samples, focusing on three key measurements: pH, bicarbonate (HCO3−) concentration, and carbon dioxide partial pressure (PCO2). This analysis follows a four-step process that helps identify the imbalance's underlying cause and nature.
    First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis).
    Next, the PCO2  and HCO3−  values are examined to...
    Disorders of Acid-Base Balance01:29

    Disorders of Acid-Base Balance

    The human body maintains a precise pH range of arterial blood between 7.35 and 7.45. Deviations result in either acidosis (pH < 7.35) or alkalosis (pH > 7.45). These conditions are further classified as respiratory or metabolic disorders based on their underlying cause.
    Respiratory Acidosis and Alkalosis
    Respiratory acidosis occurs due to an increase in the partial pressure of carbon dioxide PCO2 in the blood. It often arises from shallow breathing or impaired gas exchange caused by...
    Bronsted-Lowry Acids and Bases02:58

    Bronsted-Lowry Acids and Bases

    The acid-base reaction class has been studied for quite some time. In 1680, Robert Boyle reported traits of acid solutions that included their ability to dissolve many substances, to change the colors of certain natural dyes, and to lose these traits after coming in contact with alkali (base) solutions. In the eighteenth century, it was recognized that acids have a sour taste, react with limestone to liberate a gaseous substance (now known to be CO2), and interact with alkalis to form neutral...
    Acid-Base Balance01:25

    Acid-Base Balance

    The human body maintains a narrow pH range regulated through acid-base balance. This balance is crucial as changes in the hydrogen ion concentration can disrupt cell membrane stability, alter protein structures, and change enzyme activities. The normal pH of arterial blood is 7.4, venous blood and interstitial fluid is 7.35, and intracellular fluid averages 7.0.
    When the pH of arterial blood rises above 7.45, it results in a condition called alkalosis. Conversely, a drop below 7.35 leads to...
    Respiratory Regulation of Acid-Base Balance01:18

    Respiratory Regulation of Acid-Base Balance

    Respiratory compensation is a vital physiological process that stabilizes blood plasma pH by regulating the partial pressure of carbon dioxide (PCO2), a key determinant of pH levels. Most carbon dioxide in the blood dissolves and converts into carbonic acid (H2CO3). It dissociates into hydrogen ions (H+) and bicarbonate ions (HCO3⁻). There is also an inverse relationship between PCO2​​ and pH.
    When carbon dioxide levels increase in the blood, more H+ and HCO3⁻ are produced, leading to a...
    Renal Regulation of Acid-Base Balance01:29

    Renal Regulation of Acid-Base Balance

    Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
    In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
    However, the intercalated cells in...