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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Updated: Jun 5, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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Gene Expression Profiling via Multigene Concatemers.

Kai Jin1, Xiaoli Zheng, Yuxian Xia

  • 1Genetic Engineering Research Center, School of Bioengineering, Chongqing University, Chongqing, People's Republic of China.

Plos One
|January 27, 2011
PubMed
Summary

A new method, Multigene Concatemer Gene Expression Profiling (MgC-GEP), allows simultaneous study of multigene expression. This cost-effective technique precisely determines transcript levels for moderate-throughput analysis.

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Published on: June 27, 2017

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Studying simultaneous expression of multiple genes is crucial for understanding complex biological processes.
  • Existing methods for multigene expression profiling can be expensive or require specialized equipment.

Purpose of the Study:

  • To establish and validate a novel, cost-effective method for simultaneous multigene expression profiling.
  • To introduce Gene Expression Profiling via Multigene Concatemers (MgC-GEP) as a powerful tool for moderate-throughput analysis.

Main Methods:

  • Gene Expression Profiling via Multigene Concatemers (MgC-GEP) involves cDNA synthesis, adaptor-mediated PCR amplification, concatemer formation, cloning, and sequencing.
  • The method utilizes universal adaptors for PCR amplification and subsequent concatemer ligation.
  • Validation was performed by analyzing 20 genes in Saccharomyces cerevisiae using MgC-GEP combined with real-time RT-PCR.

Main Results:

  • MgC-GEP precisely determines transcript levels of multiple genes simultaneously.
  • The method demonstrated accuracy comparable to real-time RT-PCR and microarray analysis.
  • Validation in Saccharomyces cerevisiae confirmed the reliability of MgC-GEP.

Conclusions:

  • MgC-GEP is a novel, cost-effective, and precise method for simultaneous multigene expression profiling.
  • This technique is accessible to most laboratories, requiring no specialized equipment.
  • MgC-GEP offers a powerful solution for moderate-throughput gene expression analysis.