Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantifying intracellular mechanosensitive response upon spatially defined mechano-chemical triggering.

eLife·2026

Same author

An integrated <i>i</i> <i>n vitro</i> platform and biophysical modeling approach for studying synaptic transmission in isolated neuronal pairs.

iScience·2026

Same author

Additive Manufacturing of Nanoscale Multimaterial Voxels Via Meniscus-Confined Electrodeposition.

ACS nano·2026

Same author

Aggregation-State Dynamics Drive Double Cooperativity Between Antimicrobial Peptides LL-37 and HNP1.

Angewandte Chemie (International ed. in English)·2026

Same author

Heteroaggregation of Antimicrobial Peptides LL-37 and HNP‑1 Drives Cooperative Neutralization of Cytotoxicity.

JACS Au·2026

Same author

Inkube: an all-in-one solution for neuron culturing, electrophysiology, and fluidic exchange.

Lab on a chip·2026

Same journal

Advances in application of microplasmas for non-metallic species analysis by optical spectrometry.

The Analyst·2026

Same journal

Sulfur vacancy-mediated self-photocatalysis-boosted electrochemiluminescence sensing <i>via</i> molecular oxygen activation for sensitive detection of isocarbophos.

The Analyst·2026

Same journal

Analytical challenges in mapping the subcellular metabolome and lipidome.

The Analyst·2026

Same journal

Threshold-guided multiplex PCR-LFA: a step toward UTI pathogen detection.

The Analyst·2026

Same journal

Aptamer-based CRISPR-Cas12a fluorescent biosensors for serum biomarker detection.

The Analyst·2026

Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 5, 2026

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

Published on: December 24, 2013

Techniques for recording reconstituted ion channels.

Sophie Demarche¹, Kaori Sugihara, Tomaso Zambelli

¹Biomolecular Research, Paul Scherrer Institut (PSI), CH-5232 Villigen, Switzerland.

|January 27, 2011

Summary

This summary is machine-generated.

This review details in vitro techniques for monitoring protein ion channel activity. It covers expression, reconstitution, and analytical systems, including artificial and emerging nanotechnologies for membrane protein analysis.

More Related Videos

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Published on: January 19, 2011

Related Experiment Videos

Last Updated: Jun 5, 2026

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

Published on: December 24, 2013

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Published on: January 19, 2011

Area of Science:

Biochemistry and Biophysics
Membrane Protein Analysis

Background:

Protein ion channels are crucial membrane proteins with significant biological importance.
Studying ion channel activity requires specialized techniques for expression, reconstitution, and analysis.
Recombinant protein integration into lipid bilayers is essential for consistent experimental data.

Purpose of the Study:

To review and discuss in vitro techniques for monitoring protein ion channel activity.
To provide an overview of established and emerging analytical systems for ion channel research.
To highlight challenges and potential applications in membrane protein analysis.

Main Methods:

Focuses on analytical systems with reconstituted ion channels, briefly mentioning patch-clamp techniques.
Describes artificial systems developed over recent decades.
Presents emerging technologies utilizing nanostructured supports and microfluidic systems.

Main Results:

Outlines the biological significance and classification of ion channels.
Details various recording techniques applicable to reconstituted ion channels.
Discusses advancements in artificial and nanotechnological platforms for ion channel monitoring.

Conclusions:

In vitro techniques are vital for understanding protein ion channel function.
Artificial systems and emerging nanotechnologies offer promising avenues for enhanced analysis.
Continued development is needed to address challenges in membrane protein analysis and expand applications.