Related Experiment Video

Updated: Jun 5, 2026

Murine Model of Allergen Induced Asthma
08:05

Murine Model of Allergen Induced Asthma

Published on: May 14, 2012

ADAM-8, a metalloproteinase, drives acute allergen-induced airway inflammation

Geneviève Paulissen1, Natacha Rocks, Maud M Guéders

  • 1Laboratory of Tumor and Development Biology, GIGA-Research (GIGA-I3 and GIGA-cancer), University of Liege and CHU of Liege, Sart-Tilman, Belgium.

European Journal of Immunology
|January 27, 2011
PubMed

Insights

A disintegrin and metalloproteinase-8 (ADAM-8) promotes allergen-induced airway inflammation by increasing dendritic cell (DC) recruitment and chemokine production. Inhibiting ADAM-8 reduces inflammation in a mouse asthma model.

Area of Science:

  • Immunology
  • Respiratory Medicine
  • Molecular Biology

Background:

  • Asthma involves complex inflammatory pathways, with proteinases playing a key role.
  • A disintegrin and metalloproteinases (ADAMs) are implicated in inflammatory processes.
  • ADAM-8's specific role in allergic airway inflammation requires further investigation.

Purpose of the Study:

  • To investigate the expression and function of ADAM-8 in a mouse model of allergen-induced airway inflammation.
  • To determine the impact of ADAM-8 inhibition on inflammatory cell recruitment and mediator production.

Main Methods:

  • Utilized a mouse model of allergen-induced airway inflammation.
  • Assessed ADAM-8 expression in lung tissue and isolated dendritic cells (DCs).
  • Employed anti-ADAM-8 antibody treatment and ADAM-8 knockout mice (ADAM-8(-/-)) to evaluate functional roles.
  • Quantified inflammatory cells in bronchoalveolar lavage fluid (BALF) and peribronchial areas.
  • Measured levels of chemokines CCL11 and CCL22.

Main Results:

  • ADAM-8 expression was significantly increased in the lungs of allergen-exposed mice.
  • Treatment with anti-ADAM-8 antibody or using ADAM-8(-/-) mice reduced acute airway inflammation.
  • ADAM-8 depletion led to a significant decrease in CD11c(+) lung dendritic cells (DCs).
  • Lower levels of CCL11 and CCL22 were observed in mice with reduced ADAM-8 activity.

Conclusions:

  • ADAM-8 promotes allergen-induced acute airway inflammation.
  • ADAM-8 appears to facilitate inflammation by enhancing DC recruitment and the production of CCL11 and CCL22.
  • Targeting ADAM-8 may represent a therapeutic strategy for allergic airway diseases.

More Related Videos

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses08:16

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses

Published on: July 7, 2017

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice09:58

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

Published on: April 13, 2010

Related Concept Videos

Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
Asthma I: Introduction01:28

Asthma I: Introduction

Asthma is a chronic inflammatory disorder of the airways characterized by variable airflow obstruction and heightened bronchial responsiveness to a wide range of triggers. The underlying inflammation leads to airway swelling, mucus hypersecretion, and smooth muscle constriction, all of which narrow the airway lumen and impede airflow. Clinically, asthma presents with recurrent episodes of wheezing, shortness of breath, chest tightness, and coughing, symptoms that typically vary in intensity and...
Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
Acute Inflammation I: Inflammatory Response01:26

Acute Inflammation I: Inflammatory Response

Acute inflammation is a rapid, short-lived physiological response to tissue injury or infection, designed to eliminate harmful agents and initiate repair. This tightly regulated process typically lasts from minutes to several days and is triggered by factors such as microbial invasion, physical trauma, or chemical injury.Recognition and Mediator ReleaseThe inflammatory response begins when resident immune cells—such as mast cells, macrophages, and dendritic cells—detect damage-associated...
Allergic Reactions: Anaphylaxis01:30

Allergic Reactions: Anaphylaxis

Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin, heparin),...
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...