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Updated: Jun 4, 2026

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

Published on: April 8, 2011

Hydrostatic pressure independently increases elastin and collagen co-expression in small-diameter engineered arterial

Peter M Crapo¹, Yadong Wang

¹Department of Surgery and the McGowan Institute for Regenerative Medicine, University of Pittsburgh, 450 Technology Drive, Suite 300, Pittsburgh, Pennsylvania 15219, USA.

Journal of Biomedical Materials Research. Part A

|January 27, 2011

Summary

Applying hydrostatic pressure to engineered arterial tissues increased collagen and elastin content, enhancing burst pressure. This finding offers insights into controlling tissue properties for vascular graft development.

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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Area of Science:

Biomedical Engineering
Tissue Engineering
Cardiovascular Research

Background:

Smooth muscle cell (SMC) behavior, including proliferation, migration, and extracellular matrix production, is influenced by hydrostatic pressure in vitro.
Engineered arterial constructs offer potential for vascular graft applications.

Purpose of the Study:

To investigate the impact of hydrostatic pressure on the biological and mechanical characteristics of poly(glycerol sebacate) (PGS)-based engineered arterial constructs.
To determine how hydrostatic pressure influences collagen and elastin content, compliance, and burst pressure in these constructs.

Main Methods:

Primary adult baboon arterial SMCs were cultured on tubular, porous PGS scaffolds under pulsatile perfusion.
Hydrostatic pressure was applied using a downstream needle valve, while flow rate and pulsatility were maintained.
Constructs were assessed using pressure-diameter testing and biochemical assays for collagen and elastin content.

Main Results:

Hydrostatic pressure significantly increased construct burst pressure, collagen content, and insoluble elastin content.
Pressurized constructs showed higher soluble elastin concentration in the culture medium compared to controls.
While pressurized constructs had less elastin than native arteries, they exhibited significant elastic recovery during pressure cycling.

Conclusions:

Hydrostatic pressure is a critical factor in modulating the mechanical properties and extracellular matrix composition of engineered arterial tissues.
These findings suggest that controlled hydrostatic pressure can be utilized to optimize the development of vascular grafts with improved biomechanical characteristics.