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Identification of sunflower (Helianthus annuus, Asteraceae) hybrids using simple-sequence repeat markers
A Iqbal1, H A Sadaqat, A S Khan
1Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan. ahsanuaf2003@yahoo.com
Abstract:
Hybrid identification of 16 sunflower hybrids was confirmed using simple-sequence repeat methodology. Of 20 specific simple-sequence repeat primers, 18 authenticated the purity of these hybrids; the remaining two specific primer pairs gave ambiguous DNA fragments. The results indicate that simple-sequence repeat analysis for the identification of hybrids derived from the crossing of different inbred sunflower lines can improve the accuracy of selection, save time and reduce cost.
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02:27Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
01:18Dihybrid Crosses
01:20Monohybrid Crosses