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Updated: Jun 4, 2026

A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
Published on: December 19, 2025
Development of novel intracytoplasmic sperm injection and somatic cell nuclear transfer techniques for animal
1RIKEN Center for Developmental Biology, Chuo, Kobe, Japan. teru@cdb.riken.jp
Abstract:
Animal biotechnology has made new biological experiments possible and new discoveries are being made on an almost daily basis. Among these discoveries is a method for directly injecting a spermatozoa or somatic nucleus into an oocyte that has brought a revolution in the world of micromanipulators. Experiments that were unfeasible until now have become possible, and normal offspring can be generated from infertile cells, such as using dead sperm or a dead frozen body. In this review, I will introduce the progress of animal reproductive biotechnology, including our current research.
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