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Updated: Jun 4, 2026
Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
Published on: June 2, 2022
Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology
Stephanie Hammond1, Jeffrey C Swanberg, Mihailo Kaplarevic
1Department of Chemical Engineering, University of Delaware, Newark, DE 19711, USA.
Background:
Chinese hamster ovary (CHO) cells are among the most widely used hosts for therapeutic protein production. Yet few genomic resources are available to aid in engineering high-producing cell lines.
Results:
High-throughput Illumina sequencing was used to generate a 1x genomic coverage of an engineered CHO cell line expressing secreted alkaline phosphatase (SEAP). Reference-guided alignment and assembly produced 3.57 million contigs and CHO-specific sequence information for ~ 18,000 mouse and ~ 19,000 rat orthologous genes. The majority of these genes are involved in metabolic processes, cellular signaling, and transport and represent attractive targets for cell line engineering.
Conclusions:
This demonstrates the applicability of next-generation sequencing technology and comparative genomic analysis in the development of CHO genomic resources.
