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Automated liquid chromatographic determination of aflatoxin M1 in milk using on-line dialysis for sample preparation
L G Tuinstra1, P G Kienhuis, P Dols
1State Institute for Quality control of Agricultural Products (RIKILT), Wageningen, The Netherlands.
Journal - Association of Official Analytical Chemists
|November 1, 1990
Abstract:
A procedure has been developed for the automated isolation of aflatoxin M1 from decreamed milk. The method uses on-line stopped flow dialysis and subsequent trace enrichment on a reverse-phase column. After a back-flush to the analytical liquid chromatography column, aflatoxin M1 is determined with fluorescence detection. Fully automated analysis is possible with reproducible dialysis recoveries above 50% (CV = 7.5%, n = 25 at the 50 ng/kg level) and determination levels of 20 ng/kg within 20 min.