Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unraveling the origin of the vertebrate kidney: Emergence from a somitic compartment.

Science advances·2026
Same author

Epidermal necrolysis sequelae: A cohort study on prevalence and risk factors.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same author

Assessing the antifungal resistance of synthesized tannic acid-modified silver nanoparticles against Aspergillus niger isogenic mutants.

Fungal biology·2026
Same author

RIPOR2 promotes multinucleation of melanoma cells downstream of the RAS/ERK oncogenic pathway.

iScience·2026
Same author

Markedly reduced prevalence of Schistocephalus solidus in pelagic three-spined sticklebacks (Gasterosteus aculeatus) from Lake Constance.

Parasitology research·2026
Same author

Atopic Dermatitis-like mouse model using early inoculation of patient-derived <i>S. aureus</i> together with MC903.

JID innovations : skin science from molecules to population health·2026

Related Experiment Video

Updated: Jun 4, 2026

Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
11:55

Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

Published on: June 16, 2020

Amphioxus spawning behavior in an artificial seawater facility.

Maria Theodosiou1, Audrey Colin, Jasmin Schulz

  • 1Institut de Génomique Fonctionnelle de Lyon, Université Lyon 1, CNRS UMR5242, INRA 1288, Ecole Normale Supérieure de Lyon, Lyon, France.

Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
|January 29, 2011
PubMed
Summary
This summary is machine-generated.

Researchers successfully established laboratory maintenance and spawning protocols for amphioxus (Branchiostoma lanceolatum), a key model for vertebrate evolution. This breakthrough enables easier research on this basal chordate.

More Related Videos

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
09:31

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Published on: January 12, 2015

Incremental Temperature Changes for Maximal Breeding and Spawning in Astyanax mexicanus
06:36

Incremental Temperature Changes for Maximal Breeding and Spawning in Astyanax mexicanus

Published on: February 14, 2021

Related Experiment Videos

Last Updated: Jun 4, 2026

Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
11:55

Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

Published on: June 16, 2020

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
09:31

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Published on: January 12, 2015

Incremental Temperature Changes for Maximal Breeding and Spawning in Astyanax mexicanus
06:36

Incremental Temperature Changes for Maximal Breeding and Spawning in Astyanax mexicanus

Published on: February 14, 2021

Area of Science:

  • Developmental Biology
  • Evolutionary Biology
  • Marine Biology

Background:

  • Cephalochordate amphioxus is phylogenetically significant for understanding vertebrate evolution.
  • Lack of established animal husbandry protocols has limited amphioxus as a laboratory model.
  • Branchiostoma lanceolatum is the only amphioxus species amenable to induced spawning.

Purpose of the Study:

  • To report the first successful maintenance and spawning of Branchiostoma lanceolatum adults in a laboratory setting.
  • To provide a practical guide for establishing and managing amphioxus husbandry facilities.
  • To detail captive spawning behavior and sperm cryopreservation for amphioxus.

Main Methods:

  • Development and implementation of an artificial seawater facility for amphioxus maintenance.
  • Step-by-step protocols for facility assembly and daily husbandry operations.
  • Observation and analysis of induced and spontaneous spawning behaviors; sperm cryopreservation protocol.

Main Results:

  • Successful maintenance and induced spawning of Branchiostoma lanceolatum adults achieved.
  • Induced spawning efficiency is not sex-biased and increases with the natural spawning season.
  • Spontaneous spawning occurs in a fraction of animals, independent of induced spawning treatments or efficiency.

Conclusions:

  • Established protocols facilitate amphioxus husbandry, overcoming a major limitation for its use as a model organism.
  • This work significantly advances the utility of amphioxus in laboratory research, particularly for evolutionary developmental biology.
  • Developed protocols, including sperm cryopreservation, support the maturation of amphioxus as a key experimental model.