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Published on: July 14, 2015
Development of oleic acid-functionalized magnetite nanoparticles as hydrophobic probes for concentrating peptides
Hemei Chen1, Shasha Liu, Yan Li
1Center of Analysis and Measurement, Fudan University, Shanghai, P. R. China.
Abstract:
The oleic acid-functionalized magnetite nanoparticles (OA-Fe(3) O(4) ) with mean diameter of about 15 nm were synthesized through a low-cost, one-pot method and were designed as hydrophobic probes to realize the convenient, efficient and fast concentration of low-concentration peptides followed by MALDI-TOF-MS analysis. The capability of OA-Fe(3) O(4) nanoparticles in concentration of low-abundance peptides from simple and complex solutions were evaluated by comparing them with a sort of C8-modified magnetic microspheres. Samples of standard peptide solution, protein digest solution and human serum were introduced in the evaluating process, and the OA-Fe(3) O(4) nanoparticles exhibited good surface affinity toward low-concentration peptides.

