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Related Experiment Videos

Immunomodulation in cattle immunized with Brucella abortus strain 19.

J H Wyckoff1, A W Confer

  • 1Department of Veterinary Parasitology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078-0353.

Veterinary Immunology and Immunopathology
|December 1, 1990
PubMed
Summary

Brucella abortus Strain 19 immunization in cattle induces regulatory T lymphocytes. These cells, identified as BoCD8+ T cells, modulate the immune response and are detectable as early as seven weeks post-vaccination.

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Area of Science:

  • Veterinary Immunology
  • Bovine immunology
  • T cell regulation

Background:

  • Brucella abortus Strain 19 (S19) is a live vaccine used to control brucellosis in cattle.
  • Understanding the host immune response to S19 vaccination is crucial for optimizing vaccine efficacy and safety.
  • The role of regulatory T lymphocytes in modulating the bovine immune response to S19 is not fully understood.

Purpose of the Study:

  • To investigate the regulation of the bovine immune response following immunization with Brucella abortus Strain 19 (S19).
  • To characterize antigen-specific T lymphocyte lines derived from S19-immunized cattle.
  • To identify the cellular mechanisms involved in immune suppression after S19 vaccination.

Main Methods:

  • Application of a modified human suppressor T lymphocyte assay to bovine peripheral blood mononuclear cells (PBMC).

Related Experiment Videos

  • Development and characterization of in vitro antigen-stimulated T lymphocyte lines from S19-immunized steers.
  • Assay of culture supernates for immunomodulatory activity on indicator cell responses.
  • Surface marker expression analysis using indirect immunofluorescence and monoclonal antibodies.

    • Main Results:

    • PBMC from S19-immunized steers produced supernates that significantly suppressed indicator cell responses to mitogens and B. abortus antigens on days 35 and 49 post-immunization.
    • This suppressive activity was also induced by mitogenic stimulation (Con A, PWM) of PBMC from immunized steers.
    • Spontaneous release of suppressive activity from PBMC of immunized cattle was observed on day 49.
    • T lymphocyte lines derived from S19-immunized steers exhibited proliferative responses to B. abortus antigens and involved BoCD8+ T cells.

    Conclusions:

    • S19 immunization induces a subpopulation of regulatory cells within the PBMC of cattle.
    • This regulatory activity, involving BoCD8+ T lymphocytes specific for B. abortus antigens, is detectable as early as seven weeks post-immunization.
    • These findings provide insights into the complex immune regulation following S19 vaccination in cattle.