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Updated: Jun 4, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Development of osteogenic cell sheets for bone tissue engineering applications.

Rogério P Pirraco¹, Haruko Obokata, Takanori Iwata

¹Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.

Tissue Engineering. Part A

|February 1, 2011

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Cell sheet (CS) engineering offers a promising solution for bone tissue regeneration, overcoming limitations of traditional scaffolds. This study demonstrates successful ectopic bone formation using osteogenic cell sheets in mice.

Area of Science:

Regenerative Medicine
Biomaterials Science
Tissue Engineering

Background:

Traditional bone tissue engineering strategies using scaffolds and osteogenic cells face challenges.
Issues include biomaterial immunogenicity and poor cell viability due to nutrient diffusion limitations.
Cell sheet (CS) engineering presents an alternative approach to address these limitations.

Purpose of the Study:

To investigate the potential of osteogenic cell sheet engineering for bone regeneration.
To evaluate the efficacy of cell sheets in forming mature, vascularized bone tissue ectopically.

Main Methods:

Osteogenic cell sheets were fabricated using rat bone marrow stromal cells cultured on thermoresponsive dishes.
Cell sheets were recovered via low-temperature treatment and subcutaneously implanted into nude mice.

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Last Updated: Jun 4, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

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Published on: October 3, 2014

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

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Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Published on: October 20, 2018

Bone formation was assessed using X-ray, microcomputed tomography, and histological analysis.

Main Results:

New bone formation was observed as early as 7 days post-transplantation.
A vascularized marrow structure was evident in the newly formed bone after 6 weeks.
Histological analysis confirmed the presence of osteocytes, indicating mature bone formation.

Conclusions:

Osteogenic cell sheet engineering is a viable strategy for bone tissue regeneration.
This method overcomes key limitations of conventional scaffold-based approaches.
Cell sheet engineering shows significant potential for future bone defect repair applications.