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Amperometric determination of phosphate by use of a nucleoside phosphorylase-xanthine oxidase enzyme sensor based on
S D Haemmerli1, A A Suleiman, G G Guilbault
1University of New Orleans, Department of Chemistry, Louisiana 70148.
Analytical Biochemistry
|November 15, 1990
Abstract:
A bienzymatic sensor for the determination of phosphate was constructed by coimmobilization of xanthine oxidase (EC 1.2.3.22) and nucleoside phosphorylase (EC 2.4.2.1) on a polycarbonate membrane mounted on the tips of amperometric hydrogen peroxide and oxygen electrodes. The sensor response was linear to phosphate concentrations in the range 10-250 microM.