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Updated: Jun 4, 2026
10:01
In vitro Methylation Assay to Study Protein Arginine Methylation
Published on: October 5, 2014
Protein modification by arginylation
1Department of Biochemistry and Molecular Biology, Kimmel Cancer Center, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA, USA. Hideko.Kaji@jefferson.edu
Chemistry & Biology
|February 1, 2011
Summary
Protein arginylation, a modification catalyzed by arginyltransferases (ATE1), is a widespread biological process. This reaction is increasingly recognized as a key regulatory mechanism, similar to protein phosphorylation.
Area of Science:
- Biochemistry
- Molecular Biology
- Cellular Regulation
Background:
- Protein modification plays a crucial role in cellular signaling and function.
- Arginylation is a post-translational modification involving the addition of arginine to proteins.
- The enzyme arginyltransferase (ATE1) catalyzes the arginylation reaction.
Discussion:
- The Kashina group's research highlights the widespread occurrence of protein arginylation across biological systems.
- Arginylation is emerging as a significant regulatory mechanism in cells.
- This process is comparable in importance to well-established modifications like protein phosphorylation.
Key Insights:
- Protein arginylation is a fundamental biological process, not a rare event.
- ATE1-mediated arginylation functions as a critical regulator of protein activity and cellular pathways.
- The discovery broadens our understanding of post-translational modifications and their roles.
Outlook:
- Further research into arginylation's specific roles in various cellular processes is warranted.
- Investigating the interplay between arginylation and other post-translational modifications will be crucial.
- Exploring therapeutic applications targeting arginylation pathways could offer new avenues for disease treatment.
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02:04Covalently Linked Protein Regulators
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These groups modify specific amino acids in a protein.
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01:02Phosphorylation
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02:32Histone Modification
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Acetylation
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02:32Histone Modification
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