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Updated: Jun 4, 2026
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models
Published on: May 3, 2017
On-line microdialysis system with poly(amidoamine)-encapsulated Pt nanoparticles biosensor for glutamate sensing in
Yanyan Yu1, Qian Sun, Tianshu Zhou
1Department of Chemistry and Shanghai Key Laboratory of Green Chemistry and Chemical Process, East China Normal University, 3663 Zhongshan Road(N), Shanghai, 200062, PR China.
Abstract:
In this work, an amine-terminated poly (amidoamine) dendrimer containing Pt nanoparticles (PAMAM/Pt) nanocomposite was synthesized and a novel amperometric H(2)O(2) biosensor based on PAMAM/Pt and MWCNTs was developed. The resulting film of MWCNTs/PAMAM/Pt was characterized by transmission electron microscopy (TEM), linear sweep voltammetry (LSV) and amperometric i-t curve. It demonstrates excellent electrocatalytic responses toward the reduction of H(2)O(2) at -200 mV (vs.SCE) without HRP participation. Immobilized with glutamate oxidase (GlutaOx), an effective glutamate biosensor, was fabricated, and the in vivo detection for glutamate was realized combining with the on-line microdialysis system. The glutamate biosensor showed good linear range from 1.0 μM to 50.0 μM with the detection limit of 0.5 μM (S/N=3). The basal level of glutamate in the striatum of rat was detected continuously with this on-line system and was calculated to be 5.80±0.12 μM (n=3). This method was proved to be sensitive and selective and may be feasible in the further application of physiology and pathology.
