Performance evaluation of fast Fourier-transform continuous cyclic-voltammetry pesticide biosensor

Area of Science:

• Analytical Chemistry
• Electrochemistry
• Environmental Science

Background:

• Organophosphate pesticides (OPs) pose significant environmental and health risks.
• Rapid and sensitive detection methods for OPs are crucial for effective monitoring.
• Existing methods may lack the speed, sensitivity, or accuracy required for real-time analysis.

Purpose of the Study:

• To evaluate a novel Fast Fourier Transform Continuous Cyclic Voltammetry (FFTCCV) method for rapid organophosphate monitoring.
• To optimize experimental parameters for enhanced sensitivity and accuracy using Response Surface Methodology (RSM).
• To assess the stability, repeatability, and reproducibility of the developed analytical system.

Main Methods:

• Utilized FFTCCV with a carbon-paste electrode in a flow-injection system.
• Employed Response Surface Methodology (RSM) to optimize enzyme activity, MWCNT quantity, and sol-gel quantity.
• Determined optimal conditions including pH, flow rate, substrate concentration, and sweeping rate.

Main Results:

• Achieved a low detection limit of 1.7×10⁻⁷ mg L⁻¹ for paraoxon.
• Demonstrated long-term system stability with 80% response after 120 days.
• The optimized FFTCCV method showed high sensitivity, accuracy, and good repeatability/reproducibility.

Conclusions:

• FFTCCV is a sensitive, accurate, and fast method for organophosphate determination.
• RSM effectively optimized parameters for improved analytical performance.
• The developed flow-through biosensor is a modern tool for organophosphate pesticide analysis.