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Related Experiment Video

Updated: Jun 4, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

Microbial growth with vapor-phase substrate.

Joanna Hanzel¹, Martin Thullner, Hauke Harms

¹UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Leipzig, Germany.

Environmental Pollution (Barking, Essex : 1987)

|February 1, 2011

Summary

This study shows that bacteria can effectively degrade volatile organic contaminants (VOCs) in the vapor phase, even with limited mobility. Microorganisms act as crucial biofilters for airborne VOCs in the subsurface.

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Last Updated: Jun 4, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

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Published on: August 15, 2019

A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

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Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Published on: September 30, 2018

Area of Science:

Environmental microbiology
Subsurface science
Bioremediation

Background:

Volatile organic contaminants (VOCs) pose risks in the unsaturated subsurface.
Vapor-phase diffusion facilitates VOC transport and bioavailability.
Bacterial activity at phase boundaries is key for VOC biodegradation.

Purpose of the Study:

Investigate bacterial growth on vapor-phase naphthalene (NAPH).
Evaluate the impact of bacterial activity on vapor-phase NAPH concentrations.
Understand microbial roles in VOC biofiltration.

Main Methods:

Cultured Pseudomonas putida (NAH7) in controlled vapor-phase NAPH conditions.
Measured bacterial growth rates at varying distances from the NAPH source.
Monitored vapor-phase NAPH concentrations over time.

Main Results:

Bacterial growth rates decreased with increasing distance from the NAPH source.
Significant microbial growth occurred within 5 cm of the NAPH source.
Bacteria efficiently utilized vapor-phase NAPH, influencing concentration gradients.

Conclusions:

Suspended bacteria effectively utilize airborne VOCs.
Microorganisms play a vital role as biofilters for subsurface VOCs.
This highlights potential for bioremediation of vapor-phase contaminants.