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Related Experiment Video

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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4⁺ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Function of CD27 in helper T cell differentiation.

Sten Libregts¹, Ronald W van Olffen, Koenraad F van der Sluijs

¹Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Immunology Letters

|February 1, 2011

Summary

CD27 signaling influences T-helper cell differentiation, supporting T(H)1 cell formation but inhibiting T(H)17 cells. This effect is dependent on cytokine environment and genetic background, not providing instructive signals for specific T(H) subsets.

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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

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Published on: September 26, 2013

Area of Science:

Immunology
Cellular and Molecular Immunology

Background:

T-helper (T(H)) cell differentiation into distinct subsets is crucial for adaptive immunity.
Both cytokines and costimulatory molecules regulate T(H) cell polarization.
The role of CD27 signaling in T(H) cell differentiation remains incompletely understood.

Purpose of the Study:

To investigate whether CD27 triggering provides instructive signals for T(H)1 differentiation or broadly supports T(H) cell formation.
To determine the influence of CD27 signaling on the differentiation of various T(H) subsets (T(H)1, T(H)2, T(H)17, and regulatory T cells).

Main Methods:

Utilized CD70-transgenic (CD70TG) mice on different genetic backgrounds (C57Bl/6J and Balb/c).
Performed in vitro differentiation assays with varying cytokine milieus.
Assessed T(H) cell subset populations and transcription factor expression levels.
Induced allergic airway inflammation model in CD70TG mice.

Main Results:

CD70TG mice on a T(H)1-prone background showed increased T(H)1 cells, but this was lost on a T(H)2-prone background without a reciprocal increase in T(H)2 cells.
CD70-overexpression did not induce T(H)17 cell formation or affect regulatory T cell generation.
In vitro, CD27 triggering supported T(H)1 differentiation and inhibited T(H)17, but not T(H)2, differentiation, depending on cytokine milieu and genetic background.
In vivo, CD27 signaling supported T(H)1 differentiation in allergic airway inflammation without affecting T(H)2 responses.

Conclusions:

CD27 signaling plays a supportive, rather than instructive, role in T(H) cell differentiation.
The influence of CD27 on T(H) cell polarization is highly context-dependent, varying with cytokine environment and host genetic background.
CD27 signaling modulates T(H)1 and T(H)17 differentiation, highlighting its nuanced role in immune responses.