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Updated: Jun 4, 2026

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
08:28

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Published on: November 2, 2018

Five challenges to bringing single-molecule force spectroscopy into living cells

Yves F Dufrêne1, Evan Evans, Andreas Engel

  • 1Universite catholique de Louvain, Institute of Condensed Matter and Nanosciences, Louvain-la-Neuve, Belgium.

Nature Methods
|February 1, 2011
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...