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Updated: Jun 4, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
[Human papillomavirus genotyping of cervical uterine preneoplastic lesions in a high risk area]
Jaime López M1, Carmen Gloria Ili G, Priscilla Brebi M
1Laboratorio de Patología Molecular, Departamento de Anatomía Patológica, Facultad de Medicina/Núcleo de Desarrollo Científico Tecnológico en Biorecursos, Universidad de la Frontera, Temuco, Chile.
Background:
The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted.
Aim:
To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC.
Material And Methods:
Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped.
Results:
HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk.
Conclusions:
HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively.
