Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Physical Methods for Controlling Microbial Growth: Temperature

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...

Equipments Used to Measure Body Temperature

Equipments Used to Measure Body Temperature

Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

To Move or Not to Move: When and How Bacteria Suppress Flagellar Motility.

Molecular microbiology·2026

Same author

Heterogeneity and multi-scale dynamics in the molecular bearing of the bacterial flagellum.

Nature communications·2026

Same author

Flagellin beyond motility: exploiting flagellar filaments in biotechnology.

Trends in biotechnology·2026

Same author

A Pipeline for Generating Datasets of Three-Dimensional Tertiary Interaction Characters for Model-Based Structural Phylogenetics.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Correlation of CheY-P concentration and motor behavior during attractant adaptation in single <i>E. coli</i> cells.

Science advances·2026

Same author

Quantification of Information Flow by Dual Reporter System and Its Application to Bacterial Chemotaxis.

Physical review letters·2026

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Two methods of temperature control for single-molecule measurements.

Matthew A B Baker¹, Yuichi Inoue, Kuniaki Takeda

¹Clarendon Laboratory, Oxford University, UK.

European Biophysics Journal : EBJ

|February 1, 2011

Summary

Precise temperature control for single-molecule experiments is crucial. New methods using Peltier devices and fluid cooling chips enable accurate temperature regulation for high numerical aperture objectives, improving molecular motor studies.

More Related Videos

Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature

Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature

Published on: November 26, 2019

Thermal Measurement Techniques in Analytical Microfluidic Devices

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

Related Experiment Videos

Last Updated: Jun 4, 2026

Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature

Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature

Published on: November 26, 2019

Thermal Measurement Techniques in Analytical Microfluidic Devices

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

Area of Science:

Single-molecule biophysics
Microscopy techniques
Molecular motors

Background:

High numerical aperture (NA) oil-immersion objectives are essential for modern single-molecule biophysical experiments.
These objectives require close proximity to the sample, making precise temperature control challenging.
Temperature fluctuations can significantly affect the dynamics and function of biomolecular systems.

Purpose of the Study:

To develop and validate novel methods for precise temperature control in microscopy setups utilizing high NA objectives.
To demonstrate the applicability of these temperature control systems across different microscope platforms.
To investigate the temperature-dependent behavior of key molecular motors.

Main Methods:

Objective temperature control was achieved using a custom-designed, ring-shaped Peltier device surrounding the objective.
Stage temperature control was implemented using a fluid flow cooling chip in direct thermal contact with the sample.
The efficacy of both systems was assessed by measuring the temperature-dependent speed of molecular motors.

Main Results:

Both the objective-based Peltier device and the stage-based fluid cooling chip effectively controlled temperature.
The systems demonstrated the ability to modulate the speed of the bacterial flagellar motor in response to temperature changes.
Skeletal muscle myosin's speed was also shown to be temperature-dependent, validating the control methods.

Conclusions:

The developed temperature control methods are effective and adaptable for various microscopy setups requiring high NA objectives.
Precise temperature regulation is critical for accurate characterization of molecular motor dynamics.
These techniques provide a valuable tool for advancing single-molecule biophysics research.