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Hydroxyapatite-collagen composites. Part I: can the decrease of the interactions between the two components be a

Niccoletta Barbani¹, Elisabetta Rosellini, Caterina Cristallini

¹Dipartimento di Ingegneria Chimica, Chimica Industriale e Scienza dei Materiali, Università di Pisa, Largo Lucio Lazzarino, 56122, Pisa, Italy.

Journal of Materials Science. Materials in Medicine

|February 1, 2011

Summary

Type I acid soluble collagen (Col) interactions with hydroxyapatite (HA) were studied. Carbonate substitution in HA weakened collagen binding, suggesting a link to osteoporosis development.

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Area of Science:

Biomaterials Science
Bone Biology
Biochemistry

Background:

Bone is primarily composed of hydroxyapatite (HA) and collagen (Col).
Age-related increases in bone carbonate content are observed.
Osteoporosis is a condition characterized by decreased bone density and increased fracture risk.

Purpose of the Study:

To investigate the interactions between Type I acid soluble collagen (Col) and both carbonate-free hydroxyapatite (HA(1100)) and carbonate-rich hydroxyapatite (CHA).
To explore the potential relationship between increased bone carbonate content with aging and the development of osteoporosis.

Main Methods:

Preparation and examination of HA(1100)-Col and CHA-Col composites.
Analysis using Scanning Electron Microscopy (SEM) and Differential Scanning Calorimetry (DSC).
Fourier Transform Infrared (FT-IR) spectroscopy to elucidate molecular interactions.

Main Results:

SEM and DSC revealed stronger adhesion between Col and HA(1100) compared to CHA.
FT-IR indicated hydrogen bonding between Col peptide -NH groups and HA PO(4)(3-) with HA(1100).
FT-IR showed diminished -NH/phosphate interactions and stronger Ca(2+)/carbonate interactions in CHA-Col, leading to component separation.

Conclusions:

The substitution of phosphate ions by carbonate ions in the HA lattice weakens the binding between collagen and hydroxyapatite.
This weakened interaction, driven by carbonate presence, supports the hypothesis that carbonate substitution in HA is a significant factor in osteoporosis.
Further research is necessary to fully confirm this link.