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Updated: Jun 4, 2026
Titration of Human Coronaviruses Using an Immunoperoxidase Assay
Published on: April 28, 2008
[Development of serological detection assays for human coronavirus HKU1 infection and its application]
Wei-min Zhou1, Wen-ling Wang, Wen-jie Tan
1National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
Objective:
To express the nuclear capsid protein (N protein) and the spike protein (S protein) of HCoV-HKU1, and to develop the corresponding serum assay for antibody detection.
Methods:
The N protein of HCoV-HKU1 was expressed in E. Coli, anti-N antibody assay was established using Western Blotting with turn-based membrane. HCoV-HKU1 S protein was constructed in the eukaryotic expression plasmids, and confirmed by Western Blotting, S antibody assay was established using indirect immunofluorescence assay (IFA). We analyzed anti-S and anti-N antibody among 100 normal adult serum.
Results:
Expression of S and N protein were confirmed; 100 normal adult serum were analyzed using the established serological detection assay, in which HCoV-HKU1 S antibody positive rate was 47%, N antibody positive rate was 48%, Both S and N antibodies positive were 21%, Both S and N antibodies negative were 22%. Co-detection S and N antibody was achieved 74% positive rate.
Conclusion:
The methods we established here could be used for serological analysis of HCoV-HKU1. Either detection of HCoV-HKU1 S or N antibodies achieved good results. Higher positive detection rate of anti-S or anti-N antibody was found in the normal adults.
