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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: Jun 4, 2026

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

GC content around splice sites affects splicing through pre-mRNA secondary structures.

Jing Zhang1, C C Jay Kuo, Liang Chen

  • 1Ming Hsieh Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, USA.

BMC Genomics
|February 2, 2011
PubMed
Summary

GC content influences alternative splicing by affecting RNA secondary structures. GC-rich regions are associated with splice site usage, impacting protein diversity across species.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Related Experiment Videos

Last Updated: Jun 4, 2026

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Alternative splicing generates protein diversity from single genes.
  • RNA secondary structures are known to influence alternative splicing.
  • Genomic study investigates RNA secondary structures around splice sites in multiple species.

Purpose of the Study:

  • To investigate the role of RNA secondary structures in alternative splicing.
  • To explore the association between GC content and splice site usage.
  • To compare splicing mechanisms across humans, mice, fruit flies, and nematodes.

Main Methods:

  • Genomic analysis of RNA secondary structures around splice sites.
  • Comparative study across four species: Homo sapiens, Mus musculus, Drosophila melanogaster, and Caenorhabditis elegans.
  • Analysis of GC content and its correlation with splice site characteristics.

Main Results:

  • GC content is strongly associated with splice site usage in all studied species.
  • GC-enriched splice sites exhibit more stable RNA secondary structures.
  • GC content's effect on secondary structure stability is more significant than nucleotide order.

Conclusions:

  • GC content plays a crucial role in splice site usage, potentially mediating splicing through RNA secondary structures.
  • RNA secondary structures, influenced by GC content, are integral to alternative splicing regulation.
  • Findings highlight the conserved role of GC content in splicing across diverse species.