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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

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Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Ribosome Profiling02:24

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Related Experiment Video

Updated: Jun 4, 2026

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting
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Published on: January 23, 2013

Reprint filing: a profile-based solution.

D A Gass, R W Putnam

    Canadian Family Physician Medecin De Famille Canadien
    |February 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    A practical reprint filing system helps family physicians access medical information efficiently. This system uses a specific classification for better organization and requires regular updates to remain current.

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    Area of Science:

    • Medical Informatics
    • Family Medicine
    • Information Management

    Background:

    • Family physicians require efficient access to relevant medical information for patient care.
    • Existing information retrieval systems may lack practicality or ease of use for clinical practice.

    Purpose of the Study:

    • To introduce and evaluate a reprint filing system tailored for family physicians.
    • To demonstrate a practical coding mechanism for organizing medical reprints.

    Main Methods:

    • Development of a reprint filing system structured around practice profiles.
    • Implementation of the International Classification of Health Problems in Primary Care (ICHPPC) with supplemental categories for coding.
    • Regular maintenance, updating, and weeding of the reprint collection.

    Main Results:

    • The proposed system offers family physicians easy access to pertinent medical literature.
    • The ICHPPC classification provides a more practical coding approach compared to traditional methods like organ systems or Index Medicus.
    • The system is simple to maintain, update, and improve.

    Conclusions:

    • A practice profile-based reprint filing system enhances information accessibility for family physicians.
    • Effective organization and regular updates are crucial for the sustained utility of such systems.