Related Experiment Video
Updated: Jun 4, 2026
12:53
Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
Published on: July 6, 2014
Differential proteomic analysis of mammalian tissues using SILAM
Daniel B McClatchy1, Lujian Liao, Sung Kyu Park
1Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, United States of America.
Plos One
|February 2, 2011
Summary
This study used Stable Isotope Labeling in Mammals (SILAM) to analyze protein differences in rat liver and brain tissues. Researchers identified tissue-specific phosphorylation patterns and a key protein linked to a neurological disorder.
Area of Science:
- Proteomics
- Molecular Biology
- Biochemistry
Background:
- Differential protein expression drives organ-specific functions and can indicate tissue vulnerability in disease.
- Post-translational modifications vary across cell types and pathological states.
Purpose of the Study:
- To quantify and compare the proteome between mammalian tissues using Stable Isotope Labeling in Mammals (SILAM).
- To investigate tissue-specific phosphorylation patterns and identify differentially expressed proteins.
- To explore the utility of SILAM for differential proteomic analysis.
Main Methods:
- Utilized SILAM with (15)N-labeled rat tissue for quantitative proteomic analysis.
- Employed mass spectrometry to analyze nuclear extracts from liver and brain tissues.
- Performed pathway analysis on identified proteins and phosphorylation sites.
Main Results:
- Quantified 3742 phosphorylated peptides in liver and brain nuclear extracts.
- Identified tissue-specific kinase motifs and over 500 common protein identifications between tissues.
- Detected upregulation of the phosphoprotein ZFHX1B in the brain, a protein implicated in Mowat-Wilson syndrome.
- Revealed distinct nuclear pathways enriched in each tissue.
Conclusions:
- SILAM is an effective strategy for differential proteomic analysis of mammalian tissues.
- Findings provide a resource for understanding tissue-specific gene regulation.
- Identified specific phosphoproteins and pathways relevant to tissue function and disease.
