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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

[Fluorescence lifetime measurement based on different pump waveform].

Xiu-jun Huang1, Jian-guo Chen, Guo-ying Feng

  • 1College of Electronic &Information Engineering, Sichuan University, Chengdu 610065, China. xiujunmail@163.com

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|February 3, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel double-pulse detection method for measuring fluorescence lifetime under various pump wavelengths. This technique offers a more convenient and effective alternative to traditional deconvolution methods for laser media.

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Fluorescence Lifetime Macro Imager for Biomedical Applications
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Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Area of Science:

  • Spectroscopy
  • Laser Physics
  • Materials Science

Context:

  • Accurate measurement of fluorescence lifetime is crucial for understanding laser medium properties.
  • Traditional methods, like deconvolution, can be cumbersome and time-consuming, especially when analyzing fluorescence lifetime under varying pump wavelengths.
  • Existing theories for pump wave effects on fluorescence decay require robust experimental validation.

Purpose:

  • To analyze pump wave theory in relation to the fluorescence decay law.
  • To present a new, efficient method for measuring fluorescence lifetime under diverse pump wave conditions.
  • To validate the efficacy of the proposed double-pulse detection method.

Summary:

  • The study details a novel double-pulse detection method utilizing pump and probe pulses to measure fluorescence lifetime.
  • Experiments were conducted on neodymium glass and Cr:ZnSe crystals to assess the method's performance.
  • The method successfully measured fluorescence lifetime across visible, near-infrared, and mid-infrared regions under different pump wavelengths.

Impact:

  • Provides a convenient and effective alternative to complex deconvolution processes for fluorescence lifetime measurements.
  • Enables accurate fluorescence lifetime determination for laser media across a broad spectrum of pump wavelengths.
  • Facilitates easier characterization of laser materials and development of new laser technologies.