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The effect of MS14 on innate and cellular immune responses in BALB/c mice
Roya Yaraee1, Tooba Ghazanfari, Marzieh Eghtedardoost
1Department of Immunology, Medical School and Medicinal Plant Research Center, Shahed University, Tehran, Iran. r.yaraee@yahoo.com
Abstract:
MS14 is an Iranian natural preparation of herbal-marine source with no obvious toxicity in oral administration, which possesses anti-inflammatory and immunomodulatory effects. In this study, the effect of oral administration of MS14 on nitric oxide (NO) production of peritoneal macrophages and lymphocyte Th1 cytokines and delayed-type hypersensitivity (DTH) test in BALB/c mice were investigated. Peritoneal macrophages were cultured and NO production was measured by Griess method. Viability of macrophages was assayed by MTT (3-(4,5-dimethy-2-lthiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) test. Interleukin-2 (IL-2) and INFγ levels in supernatant of spleen lymphocytes culture were assayed by enzyme-linked immunosorbent assay kit. For DTH test the mice were immunized with sheep red blood cell and DTH was measured 24 h after the last immunization of mice. NO production of macrophages has been diminished significantly in MS14 treated group (about 40%) at the presence or absence of stimulators. Macrophage viability had no significant alteration after MS14 administration. However, interferon-γ production of lymphocytes was significantly decreased in MS14 group both at the absence or presence of concanavalin A (ConA; about 50%); IL-2 production declined about 20% at the presence of ConA. In comparison with the control group, MS14 had no statistically significant effect on DTH test. The results have pointed that MS14 may have immunomodulatory potentials at least through its decreasing effect on NO production of macrophages and level of Th1 cytokine pattern.
Insights
MS14, a natural herbal-marine preparation, reduces nitric oxide (NO) production in macrophages and decreases Th1 cytokines, suggesting immunomodulatory potential. This study investigated its effects on immune responses in mice.
Area of Science:
- Immunology
- Pharmacology
- Natural Products
Background:
- MS14 is a natural herbal-marine preparation from Iran.
- It exhibits anti-inflammatory and immunomodulatory effects with no observed toxicity.
- Understanding its impact on specific immune pathways is crucial.
Purpose of the Study:
- To investigate the effects of oral MS14 administration on nitric oxide (NO) production by peritoneal macrophages.
- To assess the impact of MS14 on Th1 cytokine levels (Interleukin-2 and Interferon-gamma) in spleen lymphocytes.
- To evaluate the effect of MS14 on the delayed-type hypersensitivity (DTH) response in BALB/c mice.
Main Methods:
- Macrophages were cultured to measure NO production using the Griess assay.
- Macrophage viability was assessed via MTT assay.
- Spleen lymphocytes were cultured to measure Interleukin-2 (IL-2) and Interferon-gamma (IFNγ) levels using ELISA.
- The DTH test involved immunization with sheep red blood cells.
Main Results:
- MS14 significantly reduced macrophage NO production by approximately 40%.
- Interferon-gamma levels decreased by about 50%, and IL-2 by 20% in MS14-treated lymphocytes.
- MS14 did not significantly alter macrophage viability or the DTH response.
Conclusions:
- MS14 demonstrates immunomodulatory potential by reducing macrophage NO production and Th1 cytokine levels.
- These findings suggest MS14 could be a candidate for modulating immune responses.
- Further research is warranted to elucidate the precise mechanisms of action.
