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Updated: Jun 4, 2026

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
Published on: November 21, 2014
[Study on polymorphism of membrane glycoprotein genes related to human platelet alloantigens]
Xiao-fei Lan1, Yan-ling Ying, Ying Liu
1Institute of Transfusion Medicine, Blood Center of Zhejiang Province; Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, 310006 PR China.
Objective:
To investigate the polymorphisms of platelet membrane glycoprotein genes related to human platelet alloantigen (HPA)-1 to 17w.
Methods:
The DNA segments of platelet membrane glycoprotein genes related to HPA-1 to 17w were amplified using author's designed primers. The amplification products were purified and directly sequenced to identify the HPA genotype and glycoprotein gene polymorphisms.
Results:
Thirteen new single nucleotide polymorphisms (SNPs) and a micro-satellite sequence were found in the glycoprotein genes from the 112 random samples, in which two SNPs (1333G/A and 1960G/A) in ITGB3 gene result in two amino acid change (V419M and E628K) on glycoprotein GPIIIa.
Conclusion:
New variants in platelet membrane glycoprotein genes were identified, which may lead to structure change of platelet membrane glycoprotein and affect the accuracy of partial HPA genotyping method.
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