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[Identification of a novel allele HLA-DRB1*1219]
Xiang-min Nie1, Yi Zhang, Yun-hai Fang
1China Marrow Donor Program HLA Typing Laboratory, Shandong Blood Center, Jinan, Shandong, 250014 PR China.
Objective:
To identify a novel HLA DRB1 allele in a Chinese leukemia family.
Methods:
A new HLA-DRB1 allele was initially detected by polymerase chain reaction-sequence specific primer and unusual reaction pattern by Luminex RSSO, then DNA sequencing was performed to identify the sequence of the novel allele.
Results:
The DNA sequencing revealed the presence of the new allele which differs from the closest matching HLA-DRB1*120201 by a single nucleotide substitution at position (341 C > T in exon 2), resulting in an amino acid change from Ala to Val at coden 85.
Conclusion:
A novel allele was confirmed by DNA sequencing and has been designated HLA-DRB1*1219 by the WHO Nomenclature Committee.
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