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Published on: July 17, 2021
[Forensic analysis of LCN DNA using sample concentration methods followed by miniSTR genotyping]
Li-Hua Gu1, Yan Dong, Chen Zhang
1Shanghai Key Laboratory of Crime Science Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China. crislh@eastday.com
Objective:
To optimize low copy number (LCN) DNA analysis methods for forensic STR genotyping.
Methods:
Two groups of DNA sample, extracted using either Magnetic bead method or Chelex-100 methods, were previously amplified with a Identifiler PCR Amplification kit, but no genotype was detected. The DNA samples were concentrated using either a drying method or the Microcon-100 method, then amplified using an miniFiler PCR Amplification kit and genotyped.
Results:
Among the 127 DNA samples, 47 samples, previously extracted using the Magnetic bead method, were genotyped with 36% success rate. Eighty samples, previously extracted using the Chelex-100 method, were genotyped with 30% success rate.
Conclusion:
The application of sample concentration methods and miniFiler kit can improve the success rate of LCN STR analysis.
