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Identification of Brucella abortus strain 19 by decreased ability to utilize erythritol as determined by gas liquid
D R Ewalt1, P F Ross, J B Payeur
1United States Department of Agriculture, National Veterinary Services Laboratories, Ames, IA 50010.
Abstract:
A method to identify Brucella abortus strain 19 by erythritol utilization using gas liquid chromatography (GLC) was developed. A total of 69 strains of B. abortus (41 virulent field strain isolates and 28 strain 19 isolates) were tested. Following incubation of the isolate with a standard amount of erythritol, the erythritol present in the cell suspension was acetylated and measured by GLC. Field strains of B. abortus utilized an average of 90.9% of the erythritol, whereas vaccine strains utilized an average of 42.4%. This difference in erythritol utilization will allow a more rapid identification of B. abortus strain 19.