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Updated: Jun 4, 2026
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CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
Published on: August 24, 2022
Bi-allelic gene targeting in mouse embryonic stem cells
Peri H Tate1, William C Skarnes
1Wellcome Trust Sanger Institute, Morgan Building, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom.
Methods (San Diego, Calif.)
|February 4, 2011
Summary
The EUCOMM and Knockout Mouse Phenotype (KOMP) programs provide conditional mouse alleles for thousands of genes. This resource accelerates functional genomics research by enabling precise gene ablation in stem cells.
Area of Science:
- Genomics
- Mouse models
- Stem cell biology
Background:
- The EUCOMM and KOMP programs have created conditional mouse alleles for nearly 10,000 genes.
- These resources are crucial for advancing functional genomics and understanding gene function.
Purpose of the Study:
- To present a method for conditional gene ablation in mouse embryonic stem cells.
- To utilize the EUCOMM and KOMP conditional resources for gene function studies.
Main Methods:
- Employing vectors and targeted clones from EUCOMM and KOMP resources.
- Generating inducible homozygous embryonic stem cells for controlled gene knockout.
Main Results:
- Demonstration of a reliable method for conditional gene ablation in stem cells.
- Creation of a precisely controlled experimental system for gene function analysis.
Conclusions:
- The developed method facilitates the use of EUCOMM and KOMP resources for functional genomics.
- Inducible homozygous cells offer a powerful tool for studying gene function in a model system.
