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Updated: Jun 4, 2026

Chromatin Isolation by RNA Purification (ChIRP)
Published on: March 25, 2012
Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric
Bharat D Reddy1, Yu Wang, Lifang Niu
1Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Abstract:
In Schizosaccharomyces pombe, the RNAi pathway is required for the formation of pericentric heterochromatin, proper chromosome segregation, and repression of pericentric meiotic recombination. Here we demonstrate that, when the activity of the histone H3 Lys 14 (H3K14) acetyltransferase Mst2 is eliminated, the RNAi machinery is no longer required for pericentric heterochromatin functions. We further reveal that reducing RNA polymerase II recruitment to pericentric regions is essential for maintaining heterochromatin in the absence of RNAi.
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