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Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
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CRMP5 (collapsin response mediator protein 5) regulates dendritic development and synaptic plasticity in the

Naoya Yamashita1, Bedrich Mosinger, Arpita Roy

  • 1Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|February 4, 2011
PubMed

Insights

Collapsin response mediator protein 5 (CRMP5) deficiency impairs cerebellar Purkinje cell development and synaptic plasticity. CRMP5 is crucial for brain-derived neurotrophic factor (BDNF) signaling, impacting neuronal structure and function.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Collapsin response mediator protein 5 (CRMP5) is abundant in the developing brain.
  • CRMP family proteins play roles in neuronal development and axon guidance.

Purpose of the Study:

  • To investigate the in vivo function of CRMP5 in cerebellar development and synaptic plasticity.
  • To elucidate the role of CRMP5 in brain-derived neurotrophic factor (BDNF) signaling.

Main Methods:

  • Generation of crmp5-deficient (crmp5(-/-)) mice.
  • Immunofluorescence studies of cerebellar Purkinje cells.
  • Electrophysiological recordings in cerebellar slices.
  • Neuronal culture and stimulation with BDNF.

Main Results:

  • crmp5(-/-) mice exhibited aberrant Purkinje cell dendrite morphology and reduced soma size.
  • Deficient long-term depression of excitatory synaptic transmission in crmp5(-/-) cerebellar slices.
  • BDNF-induced dendritic branching was attenuated in cultured crmp5(-/-) neurons.
  • CRMP5 was tyrosine phosphorylated upon coexpression with TrkB, the BDNF receptor.

Conclusions:

  • CRMP5 is essential for the proper development, maintenance, and synaptic plasticity of cerebellar Purkinje cells.
  • CRMP5 plays a significant role in BDNF signaling pathways, influencing neuronal structure and function.

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