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High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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Published on: March 3, 2015

Coding region: the neglected post-transcriptional code.

Eun Kyung Lee1, Myriam Gorospe

  • 1Laboratory of Cellular and Molecular Biology, NIA-IRP, NIH, Baltimore, MD, USA.

RNA Biology
|February 4, 2011
PubMed
Summary
This summary is machine-generated.

Scientists are discovering that the coding region (CR) of messenger RNA (mRNA) plays a key role in controlling mRNA stability and translation, expanding our understanding of gene regulation.

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Area of Science:

  • Molecular Biology
  • Gene Expression Regulation
  • Post-transcriptional Control

Background:

  • Mammalian mRNA turnover and translation are primarily associated with cis-elements in the 5' and 3' untranslated regions (UTRs).
  • Emerging evidence indicates that cis-elements within the coding region (CR) also influence mRNA fate.
  • This highlights a potential underappreciated layer of gene regulation.

Purpose of the Study:

  • To describe the mechanisms of post-transcriptional regulation mediated by CR sequences.
  • To explore the role of trans-binding factors (RNA-binding proteins and microRNAs) acting on CR elements.
  • To discuss the significance of the CR in enhancing gene expression control.

Main Methods:

  • Literature review and synthesis of current research on CR-mediated mRNA regulation.
  • Analysis of known RNA-binding proteins and microRNAs interacting with CR sequences.
  • Discussion of the implications for gene expression control.

Main Results:

  • The coding region (CR) of mRNA harbors cis-elements that regulate mRNA turnover and translation.
  • Trans-binding factors, including RNA-binding proteins and microRNAs, utilize CR sequences for post-transcriptional control.
  • CR-mediated regulation offers a more enriched mechanism for controlling gene expression.

Conclusions:

  • The coding region is an important regulatory element for mRNA fate, complementing UTR-based control.
  • RNA-binding proteins and microRNAs are key players in CR-mediated post-transcriptional gene regulation.
  • Future high-throughput technologies are expected to accelerate the study and understanding of CR-governed gene regulation.