Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Roles of exosomes and exosome-derived miRNAs in pulmonary fibrosis.

Frontiers in pharmacology·2022

Same author

Metal-polyphenol cross-linked titanium carbide membranes with stable interlayer spacing for efficient wastewater treatment.

Journal of colloid and interface science·2022

Same author

A new surgical approach for a child with acute torsion of the wandering spleen: A case report.

Asian journal of surgery·2022

Same author

Blood protein biomarkers in lung cancer.

Cancer letters·2022

Same author

Metformin attenuates osteoarthritis by targeting chondrocytes, synovial macrophages and adipocytes.

Rheumatology (Oxford, England)·2022

Same author

PLK1 promotes cholesterol efflux and alleviates atherosclerosis by up-regulating ABCA1 and ABCG1 expression via the AMPK/PPARγ/LXRα pathway.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2022

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Published on: September 2, 2022

Improved antigen cross-presentation by polyethyleneimine-based nanoparticles.

Jian Chen¹, Zhengrong Li, Hong Huang

¹School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

International Journal of Nanomedicine

|February 4, 2011

Summary

Polyethyleneimine (PEI) nanoparticles effectively deliver protein antigens for enhanced cross-presentation via the MHC I pathway. This system shows promise for developing novel cancer therapeutic vaccines by stimulating crucial immune responses.

Keywords:

cross-presentation dendritic cells polyethyleneimine vaccine

More Related Videos

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Published on: April 29, 2015

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Published on: October 12, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Published on: September 2, 2022

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Published on: April 29, 2015

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Published on: October 12, 2018

Area of Science:

Immunology
Nanotechnology
Vaccine Development

Background:

Effective therapeutic cancer vaccines require strategies to stimulate cell-mediated immunity against tumor antigens.
Antigen cross-presentation is critical for activating cytotoxic T lymphocytes against tumor cells.

Purpose of the Study:

To develop a polyethyleneimine (PEI)-based nanoparticle system for protein antigen delivery.
To enhance antigen cross-presentation through the major histocompatibility complex (MHC) I pathway.

Main Methods:

Ovalbumin (OVA) was complexed with PEI to form nanoparticles.
Nanoparticle formation was confirmed using electrophoresis, scanning electron microscopy, and photon correlation spectroscopy.
Dendritic cells were stimulated in vitro with OVA-PEI nanoparticles.

Main Results:

PEI nanoparticles significantly increased the presentation of OVA/MHC I complexes on dendritic cell surfaces.
Activated dendritic cells promoted interleukin-2 secretion, indicating specific immune cell interaction.
The 'proton sponge effect' of PEI facilitated endosomal escape, enhancing MHC I pathway presentation.

Conclusions:

PEI-based nanoparticles represent a promising delivery system for therapeutic cancer vaccines.
This system effectively promotes antigen cross-presentation, a key mechanism for eliciting anti-tumor immune responses.