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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

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Updated: Jun 4, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Recent developments in electrophoretic separations on microfluidic devices.

Stacy M Kenyon1, Michelle M Meighan, Mark A Hayes

  • 1Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ, USA.

Electrophoresis
|February 4, 2011
PubMed
Summary
This summary is machine-generated.

This review covers advances in microfluidic separation science, focusing on electrophoretic techniques for portable, rapid analysis. It highlights on-chip sample preparation and separation innovations from 2008-2010.

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Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
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Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

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Last Updated: Jun 4, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
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Area of Science:

  • Explores the intersection of separation science and microfluidics.
  • Focuses on advancements in microscale analytical devices.

Background:

  • Increasing demand for portable, fast, and low-analyte-consumption analytical devices.
  • Electrophoretic separations leverage electrokinetic properties for effective scale-down.
  • Microscale phenomena offer unique advantages over traditional techniques.

Purpose of the Study:

  • To review innovative developments in microfluidic separation science.
  • To cover advancements in electrophoretic concentration, sample preparation, and on-chip separation.
  • To provide an overview of research published between January 2008 and July 2010.

Main Methods:

  • Literature review of research combining separation science and microfluidics.
  • Focus on electrophoretic separation techniques and their microfluidic applications.
  • Analysis of innovative developments including concentration, sample preparation, and on-chip separation.

Main Results:

  • Significant progress in electrophoretic concentration techniques on microfluidic platforms.
  • Development of integrated sample preparation and conditioning methods for micro-separations.
  • Demonstration of effective on-chip separation capabilities utilizing microfluidic devices.

Conclusions:

  • Microfluidic separation science, particularly using electrophoresis, offers powerful solutions for portable analytical devices.
  • Innovations in sample preparation and on-chip separation enhance device performance and applicability.
  • The field shows rapid development, promising further advancements in analytical technology.