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Related Experiment Videos

[Separation of enriched synaptosomes, synaptic vesicles and synaptic plasma membranes].

L Franke, R Uebelhack

    Acta Biologica Et Medica Germanica
    |January 1, 1978
    PubMed
    Summary

    Researchers developed a fast, simple method to isolate intact synaptosomes, synaptic plasma membranes, and vesicles. This technique uses differential and rate zonal centrifugation for high-purity fractions, confirmed by enzyme markers and electron microscopy.

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biochemistry

    Background:

    • Synaptosomes are crucial for studying synaptic function and neurotransmission.
    • Isolating intact synaptosomes, synaptic plasma membranes, and vesicles is essential for biochemical and morphological analysis.
    • Existing methods can be time-consuming or yield impure fractions.

    Purpose of the Study:

    • To develop a rapid and simple method for separating intact synaptosomes, synaptic plasma membranes, and vesicles.
    • To obtain highly enriched fractions of these subcellular components for further study.

    Main Methods:

    • Modified differential centrifugation to obtain initial synaptosome fractions.
    • Rate zonal centrifugation in a very low-density linear sucrose gradient for further purification.

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  • Enzyme marker activities and electron microscopy for fraction characterization.

    • Main Results:

    • Successfully obtained two synaptosome fractions using differential centrifugation.
    • Achieved highly enriched fractions of synaptic plasma membranes and vesicles via rate zonal centrifugation.
    • Confirmed the purity and integrity of the isolated fractions using biochemical and ultrastructural analyses.

    Conclusions:

    • The described method is effective for rapid and simple isolation of intact synaptosomes, synaptic plasma membranes, and vesicles.
    • This technique provides high-purity fractions suitable for detailed biochemical and morphological investigations.
    • The method enhances the study of synaptic structure and function.