Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Post-Operative Complications Do Not Influence Time to Adjuvant Treatment in Breast Cancer Patients Undergoing Implant-Based Reconstructions: Pre-Pectoral Versus Sub-Pectoral.

Cancers·2026

Same author

Impact of incision type in breast cancer-conserving mastectomy: a comparative analysis of outcome.

Updates in surgery·2025

Same author

Chrysosplenetin acts as a homeostasis stabilizer with dual-function in shattering Plasmodium berghei K173 resistance to artemisinin driven by both ABC transporters and heme-ROS/GSH axis.

Parasites & vectors·2025

Same author

Exercise-induced PV network plasticity in the prelimbic cortex regulates the expression of fear memory in male mice.

Translational psychiatry·2025

Same author

Species diversity of oysters (Mollusca, Bivalvia) in the intertidal zone of Hainan Island revealed by DNA barcoding analysis.

ZooKeys·2025

Same author

Evaluating Tumor Size to Ki67 Proliferation Index Ratio for Optimizing Surgical Axillary Treatment Decisions in Breast Cancer Patients.

Cancers·2025

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Serial line scan encoding imaging cytometer for both adherent and suspended cells.

Xin Heng¹, Frank Hsiung, Amir Sadri

¹Gene Expression Division, Bio-Rad Laboratories, Hercules, California 94547, United States. xin_heng@bio-rad.com

Analytical Chemistry

|February 5, 2011

Summary

We developed a novel, low-cost imaging cytometer for high-throughput bioanalysis. This system achieves high resolution and speed, enabling rapid quantitative analysis of subcellular structures in various cell types.

More Related Videos

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Related Experiment Videos

Last Updated: Jun 4, 2026

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Area of Science:

Biotechnology
Cell Biology
Analytical Chemistry

Background:

High-content bioanalysis requires advanced imaging techniques for cellular studies.
Existing imaging cytometers can be costly and may not offer sufficient throughput.
Need for efficient platforms for quantitative subcellular analysis.

Purpose of the Study:

To introduce a new, cost-effective imaging cytometer design for high-content bioanalysis.
To enable concurrent collection of multiple fluorescence channels with high resolution and throughput.
To develop image processing routines for on-the-fly quantitative analysis of subcellular structures.

Main Methods:

Utilized a low-cost linear complementary metal oxide semiconductor (CMOS) imager operating at ≥40kHz.
Employed line-scans across cellular bodies passing through a defined line-shaped focus spot.
Developed image processing algorithms for real-time subcellular structure analysis.

Main Results:

Achieved high throughput of 1000 cells/s with concurrent multi-channel fluorescence collection.
Maintained high resolution for detailed subcellular structure imaging.
Successfully characterized the prototype system using both adherent and suspended cells.

Conclusions:

The developed imaging cytometer offers a high-resolution, high-throughput, and cost-effective solution for bioanalysis.
The platform is versatile, suitable for analyzing both adherent and suspended cells.
The system facilitates quantitative analysis of subcellular structures, advancing cell biology research.